Global identification of chemical constituents and rat metabolites of Si-Miao-Wan by liquid chromatography-electrospray ionization/quadrupole time-of-flight mass spectrometry

LU JJ, HU XW, LI P, CHEN J · Chinese Journal of Natural Medicines · 2017 July

Objective

Si-Miao-Wan (SMW), a tradiational Chinese medicinal formula used for the treatment of gout and gouty arthritis for many years. Liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) method was established to identify the multiple constituents of SMW and its metabolites in rat biological samples after oral administration.

Conclusions

A total of 48 compounds, including 21 alkaloids, 12 organic acids, 2 terpenes, 3 lactones, 2 phytosterols, and 8 other compounds, were tentatively characterized in SMW. And a total of 28 prototype compounds and 10 metabolic components were found in the drug-containing biological samples. The identified compounds and metabolites may provide helpful chemical information for further pharmacology and mechanistic researches of SMW.

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