Identification of the absorbed components and metabolites of Zhi-Zi-Da-Huang decoction in rat plasma by ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry
Zhu H, Bi K, Han F, Guan J, Zhang X, Mao X, Zhao L, Li Q, Hou X, Yin R · Journal of Pharmaceutical and Biomedical Analysis · 2015 July
DOI 10.1016/j.jpba.2015.03.043
Objective
In the present study, a sensitive and selective ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC–Q-TOF-MS) method was developed to separate and identify the absorbed components and metabolites in rat plasma after oral administration of Zhi Zi Da Huang Decoction.
Conclusions
A total of 43 compounds, including 21 prototype compounds and 22 metabolites were detected and tentatively identified. Result indicated iridoid glycosides, anthraquinones, flavanones and their related metabolites were major constituents in rat plasma.
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