Chrysanthemum morifolium contains natural bioactive compounds, including 29 types of phenolics, which display significant antioxidant activity.
The study determined the antioxidant properties of Chrysanthemum morifolium by using methanol and ethyl acetate as solvents to extract and then analyse the plant's phenolic profile. The antioxidant activity was assessed with three separate in vitro tests: a reducing power assay, a Phosphomolybdenum antioxidative power assay, and the testing of inhibition of free radical 1,1-di phenyl-2-picryl hydrazyl. The content of total phenolics and total flavonoids was measured using the Folin Ciocalteu reagent, yielding a range of values.
In relation to the results, it was established that the examined plant was a significant source of bioactive compounds with notable antioxidant properties. A total of 29 phenolics, divided into 15 phenolic acids and 14 flavonoids were identified and quantified using high-performance liquid chromatography. Ellagic acid was the most present phenolic in the methanol extract and ethyl acetate fraction of green aerial parts. Meanwhile, Hesperidin was the most common flavonoid in the flower methanol extract. The ethyl acetate fraction from the flowers showed the highest efficiency in all tested antioxidant methods, indicating that different parts of the plant can vary in their antioxidant potential.