Estimation of mandarin peel oil–induced cytotoxicity and genotoxicity in human normal fibroblast and cancerous prostate cell lines
Hussien NA, Mohamed HRH. · Toxicology Mechanisms and Methods · 2020 November
DOI 10.1080/15376516.2020.1836103
Objective
The present study was conducted to study the cytotoxicity and genotoxicity of MPO on Human normal Fibroblast (HFB4) and PC3 cell lines.
Conclusions
Citrus peel oil induced preferential cytotoxic and genotoxic effects toward cancerous PC3 with no noticeable toxic effects in normal HFB4 cells.
Critical Appraisal
Published research can offer valuable insights, but a paper’s conclusions may go beyond what its results support. Critical appraisal examines the methods, findings and limitations to clarify what can reasonably be concluded—and what remains uncertain.
This article has not yet been critically appraised by the Research Hub.
Learn more about Critical Appraisal
Insights
Mandarin peel oil induced preferential inhibition of human prostate cancer cell proliferation in a concentration-dependent manner.
The time-dependent induction of DNA breaks demonstrated in PC3 cells treated with MPO safe concentration-stimulated ROS generation and apoptotic DNA damage through increased expression of tumor suppressor p53 and Bax genes and decreased expression of Bcl2 and MDM2 genes. In contrast, non-significant changes were observed in the DNA integrity, ROS levels and expressions of the tested genes in the normal HFB4 cells treated with MPO. Thus, we concluded that MPO induced preferential cytotoxic and genotoxic effects toward cancerous PC3 with no noticeable toxic effects in normal HFB4 cells and therefore further in vivo studies are recommended to test its possible use as anticancer drugs.
