Comparative analysis of the compatibility effects of Danggui-Sini Decoction on a blood stasis syndrome rat model using untargeted metabolomics

Wu JX, Zheng H, Yao X, Liu XW, Zhu HJ, Yin CL, Liu X, Mo YY, Huang HM, Cheng B, Wu F, Chen ZN, Song FM, Ruan JX, Zhang HY, Liu P, Liang YH, Song H, Guo HW, Su ZH · Journal of Chromatography B · 2019 January

DOI 10.1016/j.jchromb.2018.12.017

Objective

Danggui-Sini Decoction (DSD) is one of the most widely used traditional Chinese medicine formulae (TCMF) for treating various diseases caused by cold coagulation and blood stasis due to its effect of nourishing blood to warm meridians in clinical use. However, studies of the mechanism of how it dispels blood stasis and its compatible regularity are challenging because of the complex pathophysiology of blood stasis syndrome (BSS) and the complexity of DSD, with multiple active ingredients acting on different targets. In this study, to understand the pathogenesis of BSS and assess the compatibility effects of DSD, an ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry-based untargeted metabolomics approach was used.

Conclusions

In this study, metabolic variations in rats with BSS and the intervention effect of DSD and its compatibility groups in BSS were investigated via UPLC-Q-TOF/MS based on untargeted metabolomics and multivariate statistical analysis. It demonstrated that metabolomics is a powerful tool for evaluating the efficacy and compatibility effects of traditional Chinese medicine (TCM).

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