Chaihu Shugan San treatment in functional dyspepsia rats mitigates mitochondrial damage and enhances gastric motility via regulation of Drp-1-mediated mitophagy in interstitial cells of Cajal.
In the methodology, forty Sprague-Dawley rats were divided into five distinct groups: control, model, mdivi-1, mdivi-1 plus Chaihu Shugan San, and Chaihu Shugan San only. A functional dyspepsia model was established among the rats through tail-clamping stimulation. The groups that received Chaihu Shugan San were administered an aqueous solution of the treatment twice daily while mdivi-1 was injected intraperitoneally every alternate week over a month-long period. Mitochondrial damage was then observed using kits and the expressions of related proteins were assessed with Immunofluorescence or Western Blot.
In the results discussion, conceptions in the model group indicated decreased superoxide dismutase and citrate synthase, leading to increased malondialdehyde and reactive oxygen species. Also, co-localization expression of LC3 and voltage dependent anion channel 1, Drp-1 and translocase of the outer mitochondrial membrane 20 significantly increased. Notably, the introduction of mdivi-1 or Chaihu Shugan San noticeably improved the above conditions, indicating that Chaihu Shugan San could enhance mitochondrial health and promote gastric motility in rats suffering from functional dyspepsia.
