Simultaneous Determination of Five Components of Chaihu-Shugan-San in Beagle Plasma by HPLC-MS/MS and Its Application to a Pharmacokinetic Study after a Single Dose of Chaihu-Shugan-San
Zhu Y, Wang H, Xue H, Zhang Y, Cheng Q, Chen L, Qiu X · Journal of Analytical Methods in Chemistry · 2020 August 19
DOI 10.1155/2020/8831938
Objective
To investigate the pharmacokinetic properties of five components of Chaihu-shugan-san in beagle plasma after oral administration by developing a reliable and sensitive high-performance liquid chromatography-tandem mass spectrometry method.
Conclusions
The study demonstrates that the validated high-performance liquid chromatography-tandem mass spectrometry method reliably and successfully measures the five analytes and can be applied to pharmacokinetic studies in beagles after a single dose of Chaihu-shugan-san.
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Insights
The study developed a reliable method to analyze and understand the behavior of five components in Chaihu-shugan-san, a traditional Chinese medicine, after administration in beagles.
The methodology involved developing a sensitive high-performance liquid chromatography-tandem mass spectrometry method to detect five components in beagle plasma after the oral administration of Chaihu-shugan-san, an ancient Chinese treatment. A specific column was used to separate the analytes while maintaining a constant temperature. Two solvents were used in a gradient elution process, and the flow rate and total measurement time were kept consistent.
The discussion around the results indicates that the developed method was successful in its application. The recovery rate of the five analytes ranged widely but remained satisfactory, as did the effects on the matrix. The study hence validates the method for studying the pharmacokinetics of these components in beagles after they've been given a single dose of Chaihu-shugan-san.
