Insights & Discussion
Gui Zhi Fu Ling Wan can potentially reduce and induce apoptosis in uterine leiomyomas cells.
In the study, human uterine myoma cells (hUtMCs) were collected from patients who had undergone surgery for this condition. The viability of these cells was tested when exposed to various doses of Gyejibongnyeong-hwan (GBH). Determination of adequate concentration for the treatment was also performed, ensuring that it did not affect normal cells adversely. Western blotting was employed to identify the expression levels of certain proteins that indicate cellular apoptosis, while the levels of reactive oxygen species were evaluated through fluorescence microscopy. Finally, the researchers looked into the time-dependent anti-neoplastic effect of GBH in order to establish an appropriate treatment duration under a non-harmful concentration of the medication.
The results showed that Gyejibongnyeong-hwan (GBH) drastically reduced the viability and number of hUtMCs within 48 hours in a dose-dependent manner. It was identified that the ratio of certain proteins and the expression of other apoptosis-related ones increased, suggesting that GBH induced cellular self-destruction in uterine leiomyomas. Moreover, it was shown that GBH noticeably increased the mitochondrial concentration of reactive oxygen species. Conversely, by using certain inhibitors along with GBH, the apoptotic effect mediated by GBH was mitigated. This evidence suggests that GBH could potentially trigger apoptosis of leiomyomas and hence, presents a promising therapeutic agent in managing uterine leiomyomas.
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Epigallocatechin gallate (EGCG), a green tea component, showed potential in treating uterine fibroids and associated infertility without causing liver toxicity or folate deficiency.
2023 Nutrients Assessing the Hepatic Safety of Epigallocatechin Gallate (EGCG) in Reproductive-Aged Women Siblini H, Al-Hendy A, Segars J, González F, Taylor HS, Singh B, et al.
Cohort Study EGCG Green Tea Uterine Fibroids
The study involved the pre-testing of a green tea substance known as Epigallocatechin gallate (EGCG) on a cohort of premenopausal women, aged 18 to 40, with and without uterine fibroids, in order to evaluate its safety. The subjects were randomized into three groups, each receiving different treatments. One group took just a daily dose of 800 mg of EGCG, one group combined this EGCG dose with 100 mg of Clomiphene citrate for five days, and the third group combined it with 5 mg of Letrozole for the same period.
The results showed that none of the subjects displayed signs of drug-induced liver injury, and all had normal serum folate levels. Hence, the findings suggest that the daily intake of 800mg of EGCG, whether consumed alone or in combination with Clomiphene citrate or Letrozole for five days, is well-tolerated and not related to any liver toxicity or folate deficiency in women of reproductive age.
Combining Crila with green tea extract (EGCG) has been found to synergistically hinder the growth of human uterine fibroid cells.
2023 F&S Science The combination of natural compounds Crila and epigallocatechin gallate (EGCG) showed enhanced antiproliferative effects on human uterine fibroid cells compared to single treatments. Bai T, Ali M, Somers B, Yang Q, McKinney S, Al-Hendy A
Network Pharmacology Crila EGCG Green Tea
In the methodological process, human uterine leiomyoma (HuLM) cells were treated with varying concentrations of Crila, either alone or combined with epigallocatechin gallate (EGCG), employing various tests. Prominently, assays were performed to examine cell proliferation, with additional analyses conducted to measure protein and gene expressions of proliferation markers.
The interaction of Crila and EGCG resulted in a notable reduction in HuLM cell viability, showing an increased inhibitory effect over singular treatments. This decrease in cell proliferation is attributed to a synergistic efficacy derived from using Crila and EGCG in combination. It was notably characterised by the reduced presence of the proliferation marker, proliferating cell nuclear antigen, both at the messenger RNA and protein levels. It was observed that the process does not induce cell apoptosis, implying that the cell viability reduction is primarily due to decreased cell proliferation.
Gui Zhi Fu Ling Wan can lessen the expression of certain proteins, which may be key to its ability to impede abnormal tissue invasion and metastasis in adenomyosis.
2022 Evidence-Based Complementary and Alternative Medicine Analysis of the Mechanism of GuizhiFuling Wan in Treating Adenomyosis Based on Network Pharmacology Combined with Molecular Docking and Experimental Verification Shi Y, Zhang C, Wang X, Wang Z, Zhang Y, Liu Z, et al.
Network Pharmacology Adenomyosis Gui Zhi Fu Ling Wan
Firstly, an approach called network pharmacology was utilized which involved identifying active components of GuizhiFuling Wan (GFW), its drug targets and disease targets through public databases, and finding shared targets. The biological function and pathway of GFW in treating adenomyosis were analyzed. Then, the interaction and strength of binding between key components and targets of GFW were verified by a method known as molecular docking. In the animal part of the study, the impact of GFW on the expression of several proteins in mice with adenomyosis was observed using different staining, protein analysis, and immunohistochemical techniques.
Through collecting and intersecting data, 26 common targets were identified, with the key active compounds being baicalein, sitosterol, and -sitosterol, and the key targets being certain proteins. Enrichment analyses showed that processes such as the positive regulation of vascular endothelial migration and signaling pathways were involved in regulating angiogenesis, invasion, and metastasis in adenomyosis. Furthermore, the molecular docking results suggested that the key active compounds had substantial binding potential with the key proteins.
In vivo analysis suggested that GFW could decrease the serum content and protein expression of certain proteins in mice with adenomyosis. These findings reinforce the hypothesis that reducing the expression of these proteins might be a significant mechanism for GFW in inhibiting the invasion and metastasis of atypical tissues associated with adenomyosis.
The majority of reproductive age women with endometriosis in Taiwan use traditional Chinese medicine, specifically Gui Zhi Fu Ling Wan, to relieve related symptoms.
2022 Frontiers in Pharmacology The Traditional Chinese Medicine Prescription Pattern of Endometriosis Patients in Taiwan: A Population-Based Study Ruei-Chi Fang, Yueh-Ting Tsai, Jung-Nien Lai, Chia-Hao Yeh, Chien-Tung Wu
Cohort Study Gui Zhi Fu Ling Wan
The methodology of this study involved analyzing the use of TCM among women with endometriosis in Taiwan. Researchers evaluated the usage, frequency of service, and the specific Chinese herbal products prescribed for endometriosis using a randomly sampled cohort of 1,000,000 beneficiaries from the National Health Insurance Research Database.
In terms of results, it was found that most women with endometriosis used TCM in dealing with their symptoms. These women were more likely to seek out TCM treatment than those without symptoms. Gui-Zhi-Fu-Ling-Wan, a Cinnamon Twig and Poria Pill known for its sedative and anti-inflammatory properties, was the most commonly prescribed Chinese herbal formula. This specific TCM plays a significant role in treating the discomfort associated with endometriosis, showing a preference towards natural remedies over conventional ones.
Green tea extract, specifically epigallocatechin gallate, can inhibit tumor growth and enhance the quality of life in women suffering from uterine fibroids.
2022 International Journal of Health Sciences The use of green tea in the treatment of symptomatic uterine fibroids Badawy A, Shady NW, Maklad SMA, Ait-Allah AS
Randomised Controlled Trial EGCG Green Tea Uterine Fibroids
A total of 75 reproductive-age women with symptomatic uterine fibroids were included in a prospective, randomized control trial. The selection of participants for either group, those receiving 900 mg of green tea extract or those receiving symptomatic treatment, was done via a random, blind method using sealed envelopes. This trial took place over a span of 4 months and the overall study lasted around 3 years.
The results of the study showcased the potency of green tea extract, particularly EGCG, with special regard to its anticancer properties. These manifested as anti-inflammatory, antiproliferative, and antioxidant effects. It was found that the administration of EGCG managed to influence tumor growth pathways, indicating its efficacy in dealing with uterine fibroids. Moreover, another significant progression was observed in the quality of life for the women partaking in this trial. It was confirmed that the use of EGCG could bring about an overall improvement in the living conditions of these women, contributing towards a better, healthier life amidst the struggle with their condition.
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