Jin Yin Hua

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Chinese Medicine Research Hub

Network Pharmacology

Honeysuckle extract could provoke anaphylaxis and specific ingredients in honeysuckle potentially trigger this reaction.

2021 Chinese Herbal Medicines Anaphylaxis effect and substance basis of honeysuckle extract
Feng Y, Qin G, Jing Z, Wang Y, Zhou Y

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A Network Pharmacology published in 2021 in the journal Chinese Herbal Medicines found that Honeysuckle extract could provoke anaphylaxis and specific ingredients in honeysuckle potentially trigger this reaction. In the methodology, rat peritoneal mast cells were separated and purified, then incubated with a compound, saline, and honeysuckle extract at 37°C for varying time intervals. Cell degranulation was observed under microscopes, and the percentage of Annexin V positive cells, which reflect cell degranulation rate, was detected via flow cytometry. Rats were given honeysuckle extract injections, and afterward, symptoms, histamine, and tryptase levels were analysed to study the anaphylaxis effect. In the discussion of results, it was discovered that the percentage of Annexin V positive cells and cell degranulation ratio significantly rose in cells treated with honeysuckle extract. Histamine and tryptase levels also increased noticeably after injection of honeysuckle extract. Regression analysis identified certain peaks as potential anaphylactoid ingredients, indicating that honeysuckle could induce anaphylaxis and there were likely specific substances in honeysuckle that caused this reaction.

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