Matcha green tea potentially inhibits the growth of breast cancer cells by disrupting metabolic action within the cells and reprogramming their activities.
Experimental Study Matcha
The research utilized MCF7 cells, a human breast cancer cell line, as a standard model for testing. To understand the effects of Matcha green tea (MGT) at the cellular level, the team conducted two sets of detailed examinations, metabolic phenotyping and proteomics analysis. Metabolic phenotyping explored the effects of the MGT on cellular metabolism, looking at its influence on both oxidative mitochondrial metabolism and glycolytic flux. Proteomics analysis identified specific proteins and enzymes which were affected by the MGT treatment.
The findings from the metabolic phenotyping displayed that MGT treatment slowed down both oxidative mitochondrial metabolism and glycolytic flux, pushing cancer cells towards a less active metabolic state. The proteomics analysis discovered specific mitochondrial proteins and glycolytic enzymes down-regulated by the introduction of MGT. Detailed bioinformatics analysis of the proteomics data revealed that the MGT drastically affected mTOR signalling, particularly down-regulating numerous components of the 40S ribosome, suggesting a possibility of MGT being used as an inhibitor of mTOR. Other pathways, including anti-oxidant response, cell cycle regulation, and interleukin signalling, were also significantly affected.
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