Herbal Formula Modified Bu-Shen-Huo-Xue Decoction Attenuates Intervertebral Disc Degeneration via Regulating Inflammation and Oxidative Stress

2022 Feb 02   Evidence-Based Complementary and Alternative Medicine   Lin J, Gu J, Fan D, Li W

The study identified 147 active ingredients and 79 common genes, including 10 core genes (TNF, VEGFA, IL6, MAPK3, AKT1, MAPK8, TP53, JUN, MMP9, and CXCL8). GO and KEGG enrichment analyses revealed MBSHXD's role in regulating inflammation and oxidative stress, particularly through pathways like the AGE-RAGE signaling pathway, IL-17 signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway, and apoptosis. The PPI network and molecular docking confirmed the interactions between nine bioactive ingredients of MBSHXD and core target proteins, supporting its "multi-component, multi-target, and multi-pathway" characteristics in treating IDD.