Insights & Discussion
Coix lacryma-jobi sprout extract effectively inhibits cervical cancer cell growth by triggering cell cycle arrest and apoptosis.
In the methodology, the research used human cervical cancer HeLa cells to investigate the effects of Coix lacryma-jobi sprout extract (CLSE). The researchers conducted a test known as Cell Counting Kit-8 assay to observe the activity of CLSE on cell multiplication. Flow-cytometric analysis and western blot analysis were also used to examine the impact of CLSE on the regulation of the cell cycle and apoptosis (cell death) in the HeLa cells.
In terms of the results, it was found that CLSE significantly halted cell proliferation, and promoted cell cycle arrest in the HeLa cells. This arrest was associated with downregulation of certain key proteins involved in cell cycle regulation. Additionally, CLSE was seen to induce apoptosis, demonstrated by specific staining techniques used to observe nuclear DNA fragmentation. The induction of apoptosis was tied to the inhibition of certain anti-apoptotic proteins and the upregulation of apoptotic proteins. Lastly, it was observed that CLSE deactivated certain key pathways in HeLa cells. As a conclusion, CLSE's therapeutic potential against cervical cancer was emphasized due to these anti-cancer effects.
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Crocin, a compound derived from saffron, has shown considerable potential in hindering tumor growth and improving immune status, across various malignant tumors.
2023 PeerJ Advances on the anti-tumor mechanisms of the carotenoid Crocin Bao X, Hu J, Zhao Y, Jia R, Zhang H, Xia L
Review Article Anti-Tumour Breast Cancer Cervical Cancer
The methodology for the study involved an in-depth review of modern pharmacological studies that have analyzed the therapeutic effects of crocin, a natural compound that can be extracted from saffron. Various anti-tumor effects were assessed including the induction of tumor cell death (apoptosis), restrictions on tumor cell proliferation, and potential barriers to invasion and metastasis of these cells. The potential for enhancement of sensitivity to chemotherapy and improvement of immune status were also examined.
Following the review, the study revealed that crocin has significant anti-tumor properties. It showed that this natural compound can induce apoptosis in tumor cells, inhibit their expansion and progression, and even prevent their invasiveness and metastasis. Furthermore, crocin demonstrated the potential to enhance the body’s responsiveness to chemotherapy and help boost the immune system. These effects were observed across a range of different malignant tumors, including stomach, liver, cervical, breast, and colorectal cancers.
The combination of epigallocatechin gallate, vitamin B12, folic acid, and hyaluronic acid could effectively counter precancerous lesions of the uterine cervix caused by HPV infections.
2023 Journal of Personalized Medicine The Association of Four Natural Molecules—EGCG, Folic Acid, Vitamin B12, and HA—To Counteract HPV Cervical Lesions: A Case Report Grandi G, Botticelli L, Fraia PD, Babalini C, Masini M, Unfer V
Case Report Cervical Cancer Cervical Dysplasia Cervical Lesions
The methodology used in this research centred around treating a 39-year-old patient who had a history of HPV, cervix lesions, and multiple failed surgical attempts to treat the condition. The treatment plan applied was unique, utilizing a blend of epigallocatechin gallate, vitamin B12, folic acid, and hyaluronic acid, administered over an eight week period.
The results showed promising effects, with the patient's histological and cytological analyses revealing only a chronic cervicitis instead of any malignant lesions or cellular dysplasia. Therefore, the necessity for invasive total hysterectomy was minimized, demonstrating the potential for the selected treatment plan to manage precancerous lesions of the uterine cervix.
Coix seed extract could augment the efficacy of gemcitabine therapy in pancreatic cancer cells at least partly due to the alteration of ABC transporter-mediated drug efflux function.
2019 International Journal of Molecular Sciences Coix Seed Extract Enhances the Anti-Pancreatic Cancer Efficacy of Gemcitabine through Regulating ABCB1- and ABCG2-Mediated Drug Efflux: A Bioluminescent Pharmacokinetic and Pharmacodynamic Study Qian, Y.; Xiong, Y.; Feng, D.; Wu, Y.; Zhang, X.; Chen, et al.
Coix Seed
Results showed that coix seed extract could synergistically enhance the anti-cancer efficacy of gemcitabine. Meanwhile coix seed extract alone or in combination with gemcitabine could significantly increase the AUCluc while decreasing the Kluc.
Western blot and immunohistochemistry assay demonstrated that coix seed extract could significantly mitigate gemcitabine-induced upregulation of ABCB1 and ABCG2 protein. The Pearson correlation analysis demonstrated that the bioluminescent pharmacokinetic parameters and pharmacodynamic index have strong association in vitro and in vivo. In conclusion coix seed extract could augment the efficacy of gemcitabine therapy in pancreatic cancer cells may at least partly due to the alteration of ABC transporter-mediated drug efflux function.
(-)-Epigallocatechingallate (EGCG), a natural product, has potent anti-proliferation, anti-metastasis, and pro-apoptosis effects on cervical cancer cells, enhancing the effects of conventional drugs.
2018 Molecules Suppressive Effects of EGCG on Cervical Cancer Wang YQ, Lu JL, Liang YR, Li QS
Review Article Anticancer Cervical Cancer Green Tea
This research paper discusses the potential of (-)-epigallocatechingallate (EGCG), a naturally occurring compound, as an anti-cancer treatment for cervical cancer. Traditional treatment methods involve the use of chemotherapy, but these have high toxicity and numerous side effects. The inherent low toxicity of EGCG makes it a promising alternative for treatment. The paper reviews the mechanisms by which EGCG inhibits the growth and spread of cervical cancer cells and promotes their apoptosis. Furthermore, the synergistic pharmaceutical effects of EGCG with conventional agents including cisplatin and bleomycin, have been evaluated.
For the discussion of results, the research shows significant evidence of EGCG's abilities to inhibit growth and spread of cervical cancer cells as well as initiate programmed cell death, or apoptosis. The compound's synergistic properties when paired with conventional chemotherapy drugs such as cisplatin and bleomycin, further enhance its potential as a treatment method. The review also explains the underlying processes by which EGCG performs these roles, providing a comprehensive view on its potential applications in cervical cancer treatment.
The major bioactive polyphenol in green tea, EGCG, could potentially reverse the abnormal changes induced by oncogenic HPV strains.
2017 The Lancet The effectiveness of epigallocathechin-3-gallate for treatment of human papillomavirus-driven epithelial neoplasms: a preclinical study Yap J, Luesley D, Woodman C, Dawson C
Cervical Cancer HPV
Methodology: This research involved organotypic raft cultures of keratinocytes infected with HPV18. The culture was established at an air-liquid interface for 10 days and then treated with EGCG for an additional 10 days. The treated raft sections were stained using antibodies specifically targeting cell proliferation, keratinocyte differentiation markers, and tumor suppressor genes. Further, western blotting was utilized on EGCG-treated cells to determine the levels of HPV18 E6 and E7 protein expression.
Results: It was observed that the EGCG treatment blocked the ability of HPV18-positive keratinocytes to produce hyperplastic epithelium within the raft culture. EGCG allowed for a decline in cell proliferation as confirmed by bromodeoxyuridine label incorporation and Ki67 staining, and it led to the upregulated expression of various tumor suppressor genes. Meanwhile, productive viral replication was impaired. Importantly, the treatment did not significantly impact keratinocyte differentiation. Rather, EGCG treatment in the culture encouraged the degradation of E6 and E7 proteins and reinstated the tumor suppressor gene expression.
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