The methodology of this study involved the use of twenty-eight Wistar rats divided into four groups: a control group, an avocado oil group given an avocado oil-based diet, an induced group, and an avocado oil testosterone-induced group. The induction of prostate hyperplasia was achieved by subcutaneously implanting silicone pellets filled with testosterone for androgen stimulation in the induced group and avocado oil testosterone-induced group. The rats were euthanized after 12 weeks, their prostates collected for analysis, prepared for paraffin processing, and stained with hematoxylin-eosin and immunostaining for p63 nuclear antigen.

The discussion of results reveals that the data showed a significant reduction in the thickness of the prostatic epithelium in the avocado oil testosterone-induced group compared to the plain induced group. This group also demonstrated a larger average alveolar area compared to the control group. More p63 in basal cells were observed in this group compared to both the avocado oil and control groups. Collagen remodeling was noted, with thicker fibers seen predominately in the control and avocado oil groups, while thinner fibers were more common in the induced and avocado oil testosterone-induced groups. These findings all suggest that avocado oil exerted a protective influence on the prostate under conditions of induced hyperplasia.